Assisted Reproduction
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Problems in the breeding process | Measures | Applicable age rage (weeks) |
Male mice of reproductive age are unwilling to mate. | In vitro fertilization + embryo transfer | ＞ 8 weeks |
Sudden death of mature male mice | Removal of the epididymis (stored at 4°C) + in vitro fertilization + embryo transfer | ＞ 8 weeks |
| Male mice with low sperm motility, small sperm, and inactive sperm. | Single sperm injection (ICSI) + embryo transfer | ＞ 8 weeks |
Death of immature male mice | Round sperm injection + embryo transfer | 3 - 8weeks |
Female mice are infertil | Ovarian transfer | ＞ 6 weeks |
| Sudden death of female mice | Ovary retrieval (stored at 4℃) + ovaria transfer | ＞ 2 weeks |
Rapid colony expansion and strain microbial purification are required. | In vitro fertilization + embryo transfer | ＞ 6 weeks |