Assisted Reproduction
Assisted reproductive techniques can recover infertile strains caused by aging, microbial infections, malformed or aberrant development of reproductive organs, and phenotypes that causes mating difficulties.
Standard assisted reproductive techniques include in vitro fertilization, intracytoplasmic single sperm injection, intracytoplasmic round sperm injection, and ovarian transplantation.
Strengths
Greater than 95% success rate restoring reproductive function in female mice.
Live offspring can be delivered to clients for further breeding.
In addition to traditional in vitro fertilization and ovarian transplantation techniques, single sperm injection and round sperm injection are alternative options based on the condition of the male mouse.
Thorough evaluation of the parental genotypes to ensure a high proportion of healthy offspring.
Depending on the many challenges encountered throughout the mouse breeding process, the following measures are available:
Problems in the breeding process | Measures | Applicable age rage (weeks) |
Male mice of reproductive age are unwilling to mate. | In vitro fertilization + embryo transfer | ＞ 8 weeks |
Sudden death of mature male mice | Removal of the epididymis (stored at 4°C) + in vitro fertilization + embryo transfer | ＞ 8 weeks |
| Male mice with low sperm motility, small sperm, and inactive sperm. | Single sperm injection (ICSI) + embryo transfer | ＞ 8 weeks |
Death of immature male mice | Round sperm injection + embryo transfer | 3 - 8weeks |
Female mice are infertil | Ovarian transfer | ＞ 6 weeks |
| Sudden death of female mice | Ovary retrieval (stored at 4℃) + ovaria transfer | ＞ 2 weeks |
Rapid colony expansion and strain microbial purification are required. | In vitro fertilization + embryo transfer | ＞ 6 weeks |